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WBPicture0000008057DescriptionFigure 2. Expression of gfp under control of the daf-1 (A-F) or daf-4 (G-K) promoters in larval and adult hermaphrodites. Labels indicate tissue types or specific cells by name (White et al., 1986). DTC, gonadal distal tip cell; PH, phasmid neurons; IL, inner labial neurons; AG, LG, VG and RVG, anterior, lateral, ventral and retrovesicular ganglia, respectively. (A) Composite of five confocal images of an L4 larva with GFP in the amphids, head ganglia, ventral cord, and ALM, PLM, PLN and PH neurons, the DTC, and a sheath surrounding the distal end of the intestine. (B,C) Nomarski interference contrast and fluorescence images of L2 larvae with GFP in neurons of the AG, LG, VG and RVG. Neural processes in the circumpharyngeal nerve ring, amphid and inner labial sensilla are marked. (D) Gonad of an L4 larva with GFP in the DTC. (E,F) Lateral and ventral views, respectively, of adult tails with GFP in PVT and five pairs of tail neurons including phasmids, PLN and PLM. (G) Head of an L1 larva with GFP in the pharynx and head ganglia. (H) Midbody of an L1 larva with GFP in the hypodermis and intestine (differences in intensity are presumably due to mitotic loss of extrachromosomal arrays in some somatic cells). (I) GFP in body wall muscle cells of an adult. (J,K) Ventrolateral and ventral views, respectively, of adult tails with GFP in PVT and two phasmid neuron pairs. Images in A,E,F,J and K were collected by krypton/argon gas laser excitation at 488 nm on a Biorad MRC-600 confocal microscope. The remaining images were reproduced in Adobe Photoshop from color slides of images produced by ultraviolet (C,G-I) or a combination of ultraviolet and Nomarski interference-contrast illumination (B,D) using a Zeiss Axioscope.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00004282