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WBPicture0000008052DescriptionFigure 5. GLH-3 and GLH-4 are P granule components. (A) GLH-3 localizes to P granules in embryos, including a four-cell embryo, a 24-cell embryo and a post-gastrulation embryo. (Inset) Two-fold embryo in which P4has divided into Z2and Z3 daughters. (B) GLH-3 protein is absentin glh-3(um1). (C) DAPI staining of B. (D) GLH-4 is in P granules in embryos; it is also found in somatic blastomeres, despite affinity purification. (E) GLH-1 (mouse antibody), GLH-2 (chicken antibody) and GLH-4 all localize to P granules in glh-3(um1) embryos. White indicates the GLHs localize together in the merged image. A-E are confocal images, except inset. (F) GLH-3 in an N2 hermaphrodite gonad surrounds each germ cell nucleus. (Inset) Proximal gonad, 3magnification of F. (G) DAPI staining of gonad in F. Germ cell nuclei (arrow),and oocytes (arrowhead) are indicated. (H) GLH-4 and GLH-1 surround the germ cell nuclei in P granules in the hermaphrodite gonad; co-localization is indicated in yellow.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00004229