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WBPicture0000008049DescriptionFigure 3. In situ hybridizations indicate glh-3 and glh-4 accumulation is specific to the adult gonad and is not localized in the early embryo. (A) glh-3 antisense RNA in the gonad of an adult hermaphrodite; arrow indicates the distal region of the gonad; insets: additional embryos from the same slide. (B) glh-3 sense RNA in an adult hermaphrodite; DAPI (blue) stains nuclei. (C) glh-4 antisense RNA in splayed young adult hermaphrodite; arrow indicates gonad; arrowhead indicates intestine, including pharynx. vit-2 probes hybridize exclusively to the intestine (not shown). (D) glh-4 antisense RNA in an isolated gonad showing mature oocyte (arrow). (E) glh-4 antisense RNA in embryos; same slide as D. Arrow: 2-cell embryo. (F) glh-4 antisense RNA in a splayed male; arrow indicates testis. The glh-3 hybridizations were 8 day exposures; glh-4 18-20 days, except for C, which was 8 days. 2x10e6 cpms of probe were used for each slide. Magnification: 400.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2000
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00004229