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| WBPicture0000008034 | Description | Figure 2. Expression of the cat-2::gfp reporter in dopaminergic cells. (A) Structure of the cat-2 (TH) locus and derived gfp reporter construct, EM#282. Hatched region is the 3UTR of unc-54, provided by the vector. (B) Rescue of cat-2(e1112) by microinjection of the cloned cat-2 gene. Dopamine (light blue), visualized by FIF treatment, is in the cell bodies of a dorsal and ventral cephalic (CEPd/v) and a deirid (ADE) neuron of the head (cells identified by position) (lateral view, anterior to left in all photomicrographs). Arrowhead indicates autofluorescence associated with gut granules. (C) CAT-2::GFP expression in head neurons in wild-type animals transformed by EM#282 (dorsal view). (D) Adult male tail showing dopamine in ray neuron cell bodies and axon terminals (FIF, lateral view). Fluorescence of the axon terminals is seen in the preanal ganglion (PAG). Yellow fluorescence is autofluorescence associated with spicules and fan structures (FIF of the socket cells of the copulatory spicules is not visible in this focal plan). (E) CAT-2::GFP in the tail of a wild-type adult male (ventral view). Fluorescence in the rays just short of the tips is consistent with localization in the dendritic endings of the A-type neurons, which, unlike the dendritic endings of the B-type neurons, do not extend into the opening at the ray tip (Sulston et al., 1980; Chow et al., 1995). Fluorescence in the body is from the cell bodies of R5A, R7A and R9A and the socket cells of the copulatory spicules (SpSo). (F) CAT-2::GFP in a dbl-1(wk70) mutant male (ventral view). Several ray morphological abnormalities are apparent: on the left side, ray 7 is fused to ray 6; on the right side, ray 5 is fused to ray 4, ray 7 to ray 6 and ray 9 to ray 8. On both sides, CAT-2::GFP is absent from rays 5 and 9 but is retained in both 6-7 fused rays. (G) CAT-2::GFP expression in multiple rays after heat-shock-induced expression of a HS::dbl-1 transgene (ventral view). A dbl-1(wk70) mutant animal carrying a mixed transgenic array generated by coinjection of the HS::dbl-1 transgene and cat-2::gfp reporter EM#282 was induced by heat shock at the Rn stage. Heat shock rescued ray 5, 7 and 9 morphology and dopaminergic identity, and induced dopaminergic identity in additional rays (compare to F). Ectopically expressing rays often display altered ray morphology; here, on both sides ray 6 has adopted a ray 4-like morphology and on the left side rays 3 and 4 are fused (3,4). All scale bars = 10 um; scale bar in G applies to D-G. | |
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| Name | FigA.jpg | ||
| Crop | Crop_picture | WBPicture0000008035 | |
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1999 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00003844 |
