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WBPicture0000007986DescriptionFigure 5. MES-6 distribution in adults and embryos. (A) Affinity-purified rabbit anti-MES-6 antibody and preimmune serum from the rabbit were used to detect proteins from a C. elegans embryo homogenate on a western blot. Adult N2 or mes mutant hermaprodites (B-F) and their embryos (G-K) were stained with affinity-purified anti-MES-6 (left) and the DNA dye, DAPI (right). (B,G) MES-6 localizes to germ nuclei, including oocytes, in the gonads of wild-type adult hermaphrodites (B). MES-6 is also faintly detectable in the larger nuclei of the gut (upper nuclei in B). MES-6 is observed in all nuclei in wild-type embryos (G). (C,H) MES-6 staining is greatly reduced or undetectable in the germlines of homozygous mes-2(bn11) mutants (C) and in their embryos (H). (D,I) MES-6 staining appears wild type in the germlines of mes-3(bn35) homozygotes (D) but is greatly reduced or undetectable in their embryos (I). (E,J) MES-6 staining appears wild type in mes-4(bn67) mutant mothers (E) and in their embryos (J). (F,K) MES-6 staining is greatly reduced or undetectable in the germlines of mes-6(bn38) homozygotes (F) and in their embryos (K). Scale bars, 50 µm (B-F) and 10 µm (G-K).
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1998
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00003145