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| WBPicture0000007981 | Description | Figure 9. Location of sma-1 RNA in C. elegans embryos by whole-mount in situ hybridization. Embryos were hybridized with pAZ4 DIG-labeled antisense probe. In all pictures anterior is to the left. (A) Early morphogenesis; lateral view, mid-focal plane, dorsal is at top of photograph. sma-1 RNA is present in cells on the posterior dorsal surface of the embryo. (B-D) Early morphogenesis (older than A); dorsal view, multiple focal planes of single embryo. (B) Upper focal plane; sma-1 RNA is present in the cells of the dorsal epidermis. (C) Mid-focal plane; sma-1 RNA is seen in the outermost layer of cells, except in the region of the head. Arrowhead, early sma-1 RNA expression in the intestine. (D) Lower focal plane; sma-1 RNA is seen in a small group of cells on the left and right side of the ventral surface (arrowheads). The position of these sma-1 expressing cells corresponds to that of the leading cells' (Williams-Masson et al., 1997), which initiate ventralward migration of the epidermis and are the first epidermal cells to reach the ventral surface. (E-H) Lateral view; mid-focal plane, dorsal is at top of photograph. By lima bean stage (E), sma-1 RNA is present on the ventral surface of the embryo (arrowhead); by comma stage (F), sma-1 RNA is present on the anterior surface of the embryo (arrowhead). sma-1 expression in pharynx and intestine is most apparent in 1.75-fold stage embryos (G); arrow marks location of boundary between pharynx and intestine. At the 2.5-fold stage (H) sma-1 RNA is not detected in the epidermis, but is still present in the intestine. Additional sma-1 expression is seen in the excretory cell (white arrowhead) and an unidentified cell near the anus (white arrow). Bar in H is 10 µm. | |
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| Name | FigD.jpg | ||
| Depict | Expr_pattern | Expr1425 | |
| Anatomy | WBbt:0003681 | ||
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| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1998 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00003094 |
