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| WBPicture0000007974 | Description | Figure 3. Micrographs showing ceh-24 reporter expression and RNA localization. Expression patterns for all constructs were assayed both in transgenic lines and in the first generation following microinjection. Identical expression patterns were observed for all constructs in both heritable lines and F1 assays. (A) ceh-24::lacZ expression in an adult animal. Expression is seen in the eight vulval muscles, eight ventral neurons in the head, and in the most posterior pharyngeal muscle, m8. (B) ceh-24::lacZ expression in the eight vulval muscles of an adult animal. (C) ceh-24::lacZ expression in the adult head. Activity is seen in pharyngeal muscle m8 and in 8-10 ventral head neurons. (D) The ceh-24 vulval muscle enhancer driving lacZ expression from a truncated pes-10 promoter in the eight vulval muscles. (E) The ceh-24 m8 enhancer driving lacZ expression from a truncated myo-2 promoter in the pharyngeal muscle m8. (F-H) In situ localization of ceh-24 RNA. Antisense probes give a pattern with ventral head neurons showing strong expression in comma stage (F), two-fold (G), and three-fold (H) embryos. No signal was seen with a comparable sense' probe (not shown). (I-L) GFP expression driven by concatamerized NdE-boxes. GFP activity is both nuclear and cytoplasmic because of the tendency of the small GFP protein to leak out of the nucleus. (I) Anal depressor muscle (black arrowhead) and the two intestinal muscles (white arrowheads) are shown. Fibers of the intestinal muscles can be seen. (J,K) Higher magnification shows cellular outline of a GFP-positive anal depressor muscle. (L) NdE-box driven GFP expression is seen in vulval and uterine muscles. Scale: Adults are 1 µm long and approximately 100 µm wide. Embryos are 50 µm long. | |
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| Name | FigA.jpg | ||
| Depict | Expr_pattern | Expr1440 | |
| Expr1441 | |||
| Anatomy | WBbt:0004292 | ||
| WBbt:0004302 | |||
| WBbt:0005342 | |||
| WBbt:0005796 | |||
| WBbt:0005821 | |||
| WBbt:0006751 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1998 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00003015 |
