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WBPicture0000007957DescriptionFigure 3. Localization of mex-1 mRNA. Photomicrographs of a wild-type hermaphrodite gonad (A) and early embryos at different developmental stages (B-H) after in situ hybridization with a probe for mex-1 mRNA; positive staining is indicated by purple (see Materials and Methods). In this and all other figures, embryos are oriented as in the schematic diagram of Fig. 1. (A) mex-1 mRNA is detected in the syncytial core of the gonad beginning in the meiotic region of the distal arm (right), and is distributed uniformly in maturing oocytes (left). mex-1 mRNA appears to be distributed uniformly in 1-cell (B) and early 2-cell (C) embryos. Beginning in late 2-cell, or 3-cell embryos (D), mex-1 mRNA appears to be at higher levels in the dividing P1blastomere than in ABor the AB daughters. (E) 4-cell embryo: mex-1 mRNA appears slightly more abundant in the germline blastomere P2(posterior) and its sister EMS (ventral) than in the AB daughters (anterior and dorsal). (F) 8-cell embryo: mex-1 mRNA can be detected at high levels in the germline blastomere P3(long arrow) and at lower levels in its somatic sister C(short arrow). (G) Early 28-cell embryo: mex-1 mRNA is detected only in the germline blastomere P4(long arrow) and its somatic sister D(short arrow). (H) Gastrulation stage embryo: mex-1 mRNA is detected in P4(long arrow), but not in D (short arrow). (I) Control 2-cell wild-type embryo stained with a sense probe for mex-1 mRNA. C. elegans embryos are about 45 µm in length.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1997
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00002661