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| WBPicture0000007955 | Description | Figure 3. SEL-1 localization. (A) Total C. elegans proteins size separated by SDS-PAGE and visualized by Coomassie blue staining. (B) shows a duplicate gel in which the proteins were transferred to nitrocellulose and probed with -SEL-1 Abs (see Materials andMethods). Full genotypes: sel-1(+) =unc-32(e189), sel-1(0) =unc-32(e189); sqt-3(sc63) sel-1(ar167). (C) Confocal micrograph of a wild-type L2 larva immunostained with anti-SEL-1 Abs: Note thepunctate staining pattern and intense staining near the lumen of the intestine, corresponding to the apical surface of these cells. sel-1 null animals prepared in parallel showed no staining (data not shown). (D) Confocal micrograph of SEL-1::GFP::SEL-1 fusion protein expressed in an L2 larva (see Materials and Methods). (E) Confocal micrograph of wild-type L3 larva immunostained with anti-SEL-1 andMH27 antibodies. | |
|---|---|---|---|
| Name | FigB.jpg | ||
| Depict | Expr_pattern | Expr549 | |
| Expr781 | |||
| Anatomy | WBbt:0005772 | ||
| Cellular_component | GO:0031410 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1997 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002660 |
