WormBase Tree Display for Picture: WBPicture0000007948
expand all nodes | collapse all nodes | view schema
| WBPicture0000007948 | Description | Figure 2. HAM-1 expression in wild-type and ham-1 mutant embryos. (A-E) Fluorescence photomicrographs of embryos stained with DAPI (blue) and a mouse anti-HAM-1 antiserum (red). (A-C, E,F) wild-type embryos, (D) ham-1 mutant embryos. (A) Earliest expression of HAM-1 begins at the 28-cell stage of development. At this stage, HAM-1 is restricted to the cell periphery to one side of dividing cells (crescent staining). The large arrow points to the asymmetrically distributed HAM-1 protein of a dividing cell and the small arrow points to the condensed chromosomes in the same cell. (B) Fluorescence photomicrograph of an embryo later in development (mid-gastrulation) that has been stained with an anti-HAM-1 antiserum. The small arrows point to two nuclei that have just formed at the end of cell division. HAM-1 protein is associated with the plasma membrane of the posterior nucleus. (C,D) Embryos at approximately 280 minutes after the division of the zygote. (C) Ventral view of a wild-type embryo. At this stage, both rings (arrowhead) and crescents (arrows) of HAM-1 protein are visible. The arrows mark the asymmetric distribution of HAM-1 in the HSN/PHB (right arrow) and ALN/PLM (left arrow) neuroblasts prior to their divisions. (D) Ventral view of a ham-1(n1438) embryo. The small arrow indicates the only detectable HAM-1 expression in this embryo. This embryo is in the same orientation and at the same stage as the wild-type embryo in C. The large arrows mark the HSN/PHB (right arrow) and ALN/PLM (left arrow) neuroblasts that are not expressing HAM-1 protein. (E) Merged image showing DAPI and anti-HAM-1 staining of the same embryo as in C. The arrow points to HAM-1 localization at the posterior cell periphery of the HSN/PHB neuroblast. This portion of the neuroblast will be inherited by the HSN/PHB precursor upon division. (F) LIN-26 staining in the same embryo as in C and E. LIN-26 is not expressed in the HSN/PHB neuroblast (arrow). The asterisk in E and F marks the position of a nucleus that is expressing LIN-26 for reference. Scale bar, 5 µm. | |
|---|---|---|---|
| Name | FigA.jpg | ||
| Crop | Crop_picture | WBPicture0000007949 | |
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1996 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002607 |
