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| WBPicture0000007947 | Description | Figure 4. Distribution of PAR-2 in early embryos. These embryos were stained with affinity purified antibody 3700 which is directed against the amino terminus of PAR-2. A fluorescein labeled secondary antibody was used. The DNA is indicated in blue via staining with DAPI. In most images the polar body is evident as a blue dot at the anterior (left) end of the egg shell. C. elegans embryos are approximately 50 µm in length.(A) Pronuclear stage embryo. The pronuclei are not completely decondensed. PAR-2 is present at the anterior and posterior poles of this embryo and absent from the lateral regions. (B) Pronuclear stage embryo. PAR-2 is restricted to the posterior cortex. (C) One-cell embryo in metaphase. The endpoints of the PAR-2 distribution have extended towards the anterior. (D) One-cell embryo in anaphase. The PAR-2 distribution is similar to that of the previous embryo. (E) The same embryo as in D. The glancing focal plane of this view shows the punctate nature of PAR-2 distribution. (F) Two-cell embryo in prophase. PAR-2 protein is restricted to the posterior cortex of P1. (G) One-cell embryofrom a hermaphrodite homozygous for the par-2(lw32) mutation. Some background staining in the cytoplasm is evident, but there is no cortical staining. (H) Four-cell embryo. PAR-2 is found all along the cortex of the P2cell. PAR-2 is also detected in the blastomere adjoining regions ofembryos at this stage. (I) 15-cell embryo doubly stained for PAR-2 and P granules. PAR-2 is seen in green and P granules are in red. The P3cell is preparing to divide and has polarized. The P granules and PAR-2 are localized to the ventral half of the cell. | |
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| Name | FigC.jpg | ||
| Remark | Expressed in the posterior cortex. | ||
| Depict | Expr_pattern | Expr579 | |
| Anatomy | WBbt:0004422 | ||
| WBbt:0006770 | |||
| WBbt:0006771 | |||
| WBbt:0006772 | |||
| WBbt:0006773 | |||
| Cellular_component | GO:0005938 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1996 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002581 |
