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WBPicture0000007945 Description: Figure 2. Localization of LIN-26 protein in embryos. Wild-type embryos were fixed and incubated with anti-LIN-26 antibodies (A,C) and visualized as described in Materials and Methods. Anterior is to the left. (A) Dorsal surface of a 350-cell embryo showing strong LIN-26 staining in all newly generated hypodermal cells and in the deirid sheath cells (ADEsh). LIN-26 protein was also detectable in the neuroblasts ABarppaapp and ABarpppapp (designated as ALM/BDU, because they are the mothers of the neurons ALM and BDU), the sisters of the hypodermal cells ABarppaapa and ABarpppapa, respectively. This staining of the ALM/BDU mothers was transient. The six arrows point to the nuclei of the bilateral XXX ADEsh cells, embryonic hypodermal cells and ALM/BDU neuroblasts, which are smaller, show less intense staining and are easily recognizable landmarks. There was no expression in the anterior part of the embryo, where are located the precursors of the support cells and head hypodermal cells. (B) Schematic drawing adapted from Sulston et al. (1983) showing the positions of the dorsal hypodermal nuclei (black nuclei) in a 350-cell embryo; the XXX and ADEshnuclei are shown in grey. The ALM/BDU nuclei are cross-hatched. (C) Lateral surface of a comma-stage embryo showing LIN-26 expression in hypodermal cells, glial-like support cells in the head (smaller nuclei; three white arrows) and hypodermal cells in the tail (single large white arrow). Other large nuclei in the head (four black arrows) and the body region (unlabeled nuclei) correspond to hypodermal cells generated at 240 minutes of development (the positions of the seam nucleus H1 and of the ADEsh nucleus are shown for orientation). (D) Schematic drawing adapted from Sulston et al. (1983) showing the positions of the lateral nuclei in a comma-stage embryo. Hypodermal nuclei (black), sheath and socket nuclei (grey). The embryo shown in C had been slightly flattened at mounting such that the hyp7 nucleus found immediately anterior to P1/2L, V1, H2 and the two hyp7 nuclei found dorsally to V1 and H2 were out of focus, while the G2 nucleus, six dorsal and six ventral head hypodermal nuclei (black arrows in panel C) and several additional tail hypodermal nuclei can be seen in C but are not indicated in the drawing shown in D.
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  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1996
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00002551