WormBase Tree Display for Picture: WBPicture0000007944

WBPicture0000007944 Description: Figure 1. Localization of LIN-26 protein in larvae and adults. Wild-type animals were stained with anti LIN-26 antibodies (A,C,E,G,I,J), the monoclonal antibody MH27 (H) or DAPI (B,D,F,K), as described in Materials and Methods. Anterior is to the left, and dorsal is up (except for the animals in C/D and G/H, which are ventral side up). (A) Ventral and left lateral anterior part of a young L1 animal (from the nerve ring to the V1 seam cell) showing LIN-26 protein in cells of the excretory system (excretory cell, excretory duct cell, G1, G2, W), the amphid sheath (AMsh, slightly out of focus) and the deirid sheath (ADEsh) cells, the seam cells H1, H2 and V1, the main hypodermal syncytium hyp7 (closed triangles), the OLL sheath, CEP socket and CEP sheath cells (open triangles from left to right, respectively). (B) DAPI staining of the animal in A (arrows and triangles point to nuclei stained in A). (C) 6-hour old L1 larva showing LIN-26 protein in Z1 and Z4, the somatic gonad precursor cells. (D) DAPI staining of the animal in C. This picture was taken using fluorescence and Nomarski optics to visualize nuclei and the gonad (arrows point at Z1 and Z4; triangles point at germline nuclei). (E) Middle focal plane of the posterior part of the L1 larva in A, showing LIN-26 protein in cells of the anus (B, F, and slightly out of focus Y, U), the rectum (K; its contralateral homolog Kis not inthis focal plane), the left seam cell T (slightly out of focus), the left phasmid sheath cell (PHsh; slightly out of focus) and in a nucleus of the main hypodermal syncytium hyp7 (Cpappd). (F) DAPI staining of the animal in E (arrows point to nuclei stained in E). (G) Ventralsurface of an 8-10 hour old L1 larvae, showing LIN-26 protein both in the anterior neuroblasts Pn.a and in the posterior hypodermoblasts Pn.p. The 12 Pn cells divide mostly in sequence, with P1 first and P12 last (Sulston and Horvitz, 1977). In this animal, in which P1-P6 had divided, LIN-26 protein appeared to be as abundant in P4.a as it is in P4.p, but more abundant in P4.a than in P3.a, in P3.a than in P2.a, and in P2.a than in P1.a, in which it was barely detectable. Conversely, the amount of the LIN-26 protein seemed higher in P1.p than in P4.p. The LIN-26 protein can also be seen in P5.a and P6.a but not in P5.p and P6.p, which are slightly out of focus. (H) Same larva as in G stained with the anti-adherens junction monoclonal antibody MH27 (Waterston, 1988). An adherens junction surrounds the P cells and their daughters, the Pn.a and Pn.p cells. The adherens junction that surrounds the Pn.a cells rapidly disappears. The adherens junction that surrounds P1.p and P2.p also disappears when they fuse with the main hypodermal syncytium. (I) Ventral and left lateral central body region of an L3 larva, showing LIN-26 protein in cells derived from the Pn.p hypodermoblasts (identified by lineages), seam cells and nuclei of the main hypodermal syncytium hyp7 (all other nuclei). (J) Region surrounding the right postdeirid of a young adult showing LIN-26 protein in the postdeirid socket (PDEso) and sheath (PDEsh) cells and in the surrounding seam cells and hyp7 hypodermal syncytium (all other nuclei). (K) DAPI staining of the animal in J. The lineage of V5.pa that generates the postdeirid is shown. Note that the postdeirid neurons (arrowheads) are not stained.
  Name: Figure1.jpg
  Depict: Expr_pattern: Expr87
      Expr587
    Anatomy (37)
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1996
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00002551