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| WBPicture0000007943 | Description | Figure 9. Hypodermal expression of lin-29::lacZ reporter fusions. Strains transgenic for lin-29A::lacZ and lin-29B::lacZ were assayed for -galactosidaseaccumulation by indirect immunofluorescence, as described in Materials and Methods. Both constructs program hypodermal expression beginning during the L2 stage. (A) lin-29 gene structure. The horizontal line depicts the lin-29 locus with respect to EcoRI restriction sites (RI). The lin-29A and lin-29B transcripts are shown above this line. The boxes represent exons. Filled boxes are protein coding regions. lin-29A exons are numbered 1-11. Exon 1 of lin-29A is trans-spliced to SL1. The lin-29B-specific exon is referred to as exon 1b. The remaining exons are shared with lin-29A and are referred to as exons 5b-11b. cDNA analysis has revealed that lin-29B can also be trans-spliced to SL1 at the start of exon 5b (Rougvie and Ambros, 1995). lin-29 gene fragments used in lacZ reporter gene constructs are shown below the restriction map (see Materials and Methods). (B) A L3 larva transgenic for lin-29A::lacZ and stained for beta-galactosidase and MH27. | |
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| Name | Figure9.jpg | ||
| Depict | Expr_pattern | Expr1596 | |
| Anatomy | WBbt:0005753 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1996 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002521 |
