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WBPicture0000007942DescriptionFigure 6. Accumulation of LIN-29 in non-hypodermal cells. Left panels, wild-type worms stained with anti-LIN-29 antibodies. Right panels, corresponding focal plane showing Hoechst-staining nuclei. (A-B) Mid-section focal plane through the head of a wild-type L4 molt animal. Many non-hypodermal nuclei in the head accumulate LIN-29, particularly in the metacorpus (m) and the terminal bulb (tb) of the pharynx. (C-D) Tail of a wild-type animal during the L4-molt. The rectal cells B, F and U accumulate LIN-29. Several tail hypodermal nuclei, including hyp12 and K.a, have accumulated LIN-29 and are also visible in this focal plane. (E-F) The mid-region of a wild-type early L4-stage animal. Ventral is down. The eight left sex muscle cell nuclei accumulate LIN-29 and are indicated by vertical lines. The eight sex muscle cells on the right side of the worm also accumulate LIN-29. LIN-29 first appears in this lineage during the L3 stage. Two seam cell nuclei that are present in this focal plane are indicated by arrowheads. Vulval cell nuclei present on the ventral side of the worm also accumulate LIN-29 and are indicated by a bracket. (G-H) The mid-portion of a wild-type L3 molt-stage worm. Early during the L3 stage, the anchor cell (AC) nucleus accumulates LIN-29. A short time later the two distal tip cell nuclei (DTC), present at the tips of the developing gonad, accumulate LIN-29. The cell nuclei of the developing vulva also accumulate LIN-29 and are visible in this focal plane (bracket). (I-J) Heads of two wild-type L2-stage animals. LIN-29 accumulation is mainly in pharyngeal cell nuclei. LIN-29 is present in these nuclei at low, but detectable, levels beginning during the L1 stage, and appears to increase in abundance as development proceeds to the L4 stage. Hypodermal accumulation of LIN-29 is not observed in the head prior to the L4 stage.
NameFigure6.jpg
DepictExpr_patternExpr1596
Anatomy (11)
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1996
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00002521