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| WBPicture0000007941 | Description | Figure 3. Accumulation of LIN-29 during development. (A) The wild-type cell lineage pattern is shown for V1-V4 and V6 (Sulston and Horvitz, 1977). The seam cells that accumulate LIN-29 are indicated by open circles. (B) Wild-type L4-stage hermaphrodite stained with anti-LIN-29 antibodies. LIN-29 accumulates in seam cell nuclei positioned along the lateral seam (open circles in A). The worm is curled, with anterior to the left. The row of tightly spaced nuclei that have accumulated LIN-29 are the progeny of the sex myoblasts (see Fig. 6E). (C) Wild-type L3-stage hermaphrodite co-stained with anti-LIN-29 antibodies and MH27. MH27, in conjunction with the Hoechst staining (D), unambiguously identifies the lateral seam cells and their nuclei (arrowheads). LIN-29 is not detected in the L3 seam. (E) Wild-type L4-stage hermaphrodite co-stained with anti-LIN-29 antibodies and MH27. LIN-29 accumulates in L4-stage seam cell nuclei (arrowheads). The asterisk indicates the nucleus of a cell that is about to fuse to hyp7, and has not yet accumulated LIN-29. (F) Hoechst staining in the same focal plane. (G) The head of a wild-type animal during the L4-molt stained with anti-LIN-29 antibodies. Many nuclei in the head accumulate LIN-29 during the L4 stage. This includes all of the hypodermal nuclei, a subset of which are indicated. All of the nuclei that fuse into hyp7 (marked hyp7 in A) accumulate LIN-29 during the L4 stage. A subset are indicated in this figure. Nuclei of the pharynx, particularly the metacorpus (m) and terminal bulb (tb), accumulate LIN-29 (see also Fig. 6A). (H) Hoechst staining in the same focal plane. (I) The tail of a wild-type animal during the L4 molt stained with anti-LIN-29 antibodies. All nuclei of the tail hypodermis [hyp8-hyp12 (hyp12=P12.pa; Sulston et al., 1988); K.a; see Fig. 6C] accumulate LIN-29. Two nuclei of hyp7 are also present in this focal plane. (J) Hoechst staining of the same focal plane. (K) A wild-type animal during the L4-molt stained with anti-LIN-29 antibodies. Two rows of nuclei from hyp7 and the seam are visible in this focal plane and are indicated. m and tb, metacorpus and terminal bulb of the pharynx, respectively. LIN-29 accumulation in the vulval region (out of the plane of focus) is also indicated. | |
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| Name | Figure3.jpg | ||
| Depict | Expr_pattern | Expr1596 | |
| Anatomy | WBbt:0005734 | ||
| WBbt:0005753 | |||
| Cellular_component | GO:0005634 | ||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1996 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002521 |
