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WBPicture0000007939DescriptionFigure 2. LIN-29 is a nuclear protein. (A) Diagrams of the two lin-29 predicted proteins are shown. LIN-29A is a 459-amino acid protein. LIN-29B is identical except for the lack of the N-terminal 142 amino acids. Zn indicates the position of each zinc finger. The 86-amino acid C-terminal domain included in the GST::LIN-29 fusion protein shown below is indicated by a filled box. The position of the premature stop in lin-29(n546) is indicated by a vertical line. (B-E) Arrowheads indicate the positions of lateral hypodermal seam cell nuclei. Unless otherwise noted, anterior is to the left in all figures and left lateral views are shown. (B) Wild-type adult stained with anti-LIN-29 and MH27 antibodies and visualized by indirect immunofluorescence. LIN-29 has accumulated in the seam cell nuclei. MH27 outlines the fused hypodermal seam, which is seen as two parallel lines. (C) Hoechst staining of worm in B to show nuclei. (D) lin-29(n546) mutant adult stained with anti-LIN-29 and MH27 antibodies. LIN-29 is not detected. Even though this is an adult, based upon gonadal development, the MH27 staining indicates that the seam cells have failed to fuse. (E) Hoechst staining of worm shown in D.
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CropCrop_pictureWBPicture0000007940
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1996
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00002521