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WBPicture0000007911 | Description | Figure 6. Distribution of LAG-2::β-gal and GLP-1 in adult germline.(A) Schematic representation of distal region of adult germline, showing DTC on left. DTC nucleus shown as open circle, mitotic germ nuclei shown as black circles. Thin lines represent cell membranes, mitotic germ line is a syncytium. Scale bar, 20 µm. (B-D) Confocal images of dissected germlines that have been double stained with both anti-β-galactosidase (red) and anti-GLP-1 (green)antibodies. (B) GLP-1 is present in germline membranes. See Crittenden et al. (accompanying paper) to see definitively that the DTC does not contain GLP-1. (C) The LAG-2::β-gal fusion protein is present in the DTC and also near the center of the germline. (D) Simultaneous detection of anti-GLP-1 and anti-β-gal using merged fluorescein and rhodamine confocal image. Note co-localization of GLP-1 staining and β-gal staining within membranes of the germline. | |
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Name | FigH.jpg | ||
Depict | Expr_pattern | Expr1578 | |
Anatomy | WBbt:0004506 | ||
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
Publication_year | 1994 | ||
Journal_URL | Development | ||
Publisher_URL | TheCompanyofBiologists | ||
Reference | WBPaper00002046 |