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WBPicture0000007902 Description: Figure 2. Expression of the mab-5-lacZ fusion during embryonic development. Staining is localized to the nuclei due to a nuclear localization signal in the fusion protein (Salser and Kenyon, 1991). Times indicated are normalized to development at 20 C. Anterior is to the left except in E, as noted. A, C, F and I are tracings of the embryos shown in B, D, G and J, with the stained cells outlined. (A andB) 180 minutes, ventral view, superficial plane. Earliest expression of the fusion. Two bilateral pairs of cells, ABplpppp(a/p) and ABprpppp(a/p), are the only cells stained at this stage. Gastrulation is in progress, and the ventral cleft can be seen (arrowhead). (C and D) 270 minutes, ventral view, superficial plane. The 8 granddaughters of ABplpppp and ABprpppp are visible in this focal plane. The polar body is indicated with an open arrowhead. (E) A diagram of the cells identified as staining at 270 minutes, ventral view, anterior to the top. The drawing is from Figure 7B of Sulston et al. (1983), with lacZ-expressing cells colored in. Stippled cells are cell deaths. At this stage, at least 22 cells are stained, including: 8 descendants of ABplpppp and ABprpppp [These cells will divide again and give rise to the following cells: PVP (L and R), rectal epithelial cell V (L and R), F, U, DVA, B, an anal depressor muscle, intestinal muscle, sphincter muscle, and a body muscle, 2 hyp10 nuclei, and 2 tail spike cells.]; 6 ventral hypodermal cells (P7/8, P9/10, and P11/12 on each side); two cells, one next to V6 on each side, which we believe to be ABplapapp and ABprapapp (the sisters of P11/12 L and R, which give rise to ALN and PLM on each side); the two lateral hypodermal cells V6L and V6R; the dorsal hypodermal cells Caaapp and Cpaapp; and two posterior intestinal cells (not indicated). It is possible that additional cells within the region are stained and have not been identified. (F, G and H) 350 minutes, lateral view, superficial plane. The embryo has just begun elongation. Ventral hypodermal cells P7/8, P9/10, and P11/12, and the lateral hypodermal cell V6 (arrows) are visible staining in this plane of focus. H shows DAPI staining; the row of lateral hypodermal (V) nuclei (upper row of nuclei) and ventral hypodermal (P) nuclei are visible lined up along the side of the embryo. (I and J) 430 minutes (1 -fold), left lateral view, superficial plane. Ventral hypodermal cells P7/8, P9/10, P11/12, the lateral hypodermal cell V6, and the dorsal hypodermal nucleus Cpaapp (arrows) are visible. (K) 430 minutes, left lateral view, mid-focal plane (a different embryo than in Iand J) Staining of ventral motor neuron precursors is visible. An unidentified stained cell behind the anus is also seen in this focal plane. Embryos in B, D, G and H were muIs2 unc-31(e169); embryos in J and K were muIs3. No differences were seen in the staining pattern of the two strains at these stages.
  Name: FigB.jpg
  Depict: Expr_pattern: Expr585
    Anatomy (22)
    Cellular_component: GO:0005634
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1992
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00001639