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| WBPicture0000007471 | Description | Figure 3. ife-1 mRNA is enriched in the germline. (A) Northern analysis of ife-1 mRNA. Poly(A)+ RNA was prepared from synchronous populations of wild-type (N2) and glp-4(bn2ts) hermaphrodites grown at 25 C, at which temperature glp-4 mutants produce a severely underproliferated germline. The rpp-1 mRNA, which encodes a ribosomal protein, was used as a loading control. Relative levels of ife-1 mRNA are shown at the bottom. (B) Distribution of ife-1 mRNA in embryos, as revealed by whole-mount in situ hybridization. The cDNA clone yk504h9 was used as a probe. ife-1 mRNA is present in all cells until the 100-200-cell stage and gradually disappears by the 300-400-cell stage. (C) Distribution of ife-1 mRNA in larvae and adult hermaphrodites. ife-1 mRNA first becomes detectable in the germline of L3 stage larvae and is maintained in the germline throughout adulthood. The signal is more intense in the regions of the gonad undergoing spermatogenesis (carets). | |
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| Name | F3.large.jpg | ||
| Depict | Expr_pattern | Expr1674 | |
| Expr2324 | |||
| Anatomy | WBbt:0005175 | ||
| WBbt:0005784 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004940 |
