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| WBPicture0000007445 | Description | Figure 6. Expression patterns of elt-5 and -6 reporters and endogenous ELT-5 and -6 proteins. (A,B) Expression of the elt-5 translational fusion, pKK52. (A) Ventral view of a 28-cell stage embryo. GFP is seen in the nuclei of all four MS granddaughters (arrowheads) and all 16 AB great great granddaughters. Only 14 AB descendants are visible in this focal plane. (B) Lateral view of a comma-stage embryo. Strong GFP expression is present in seam cells and several cells in the head, tentatively identified as neurons and/or neuronal support cells. Somewhat weaker expression is observed in many other cells, mostly in the head and tail regions; these are likely to be descendants of the AB and MS founder cells. (C,D) Embryos at the 1.5-fold (C) and threefold (D) stages stained with anti-ELT-5 (red) and MH27 (green). High levels of ELT-5 are detected in all seam cells and in many other cells in the head and tail regions at these stages. (E,F) Expression patterns of the elt-6 transcriptional fusion reporter, pKK41. (E) Embryo at the 1.5-fold stage shows strong GFP expression in several cells in the head region, tentatively identified as neurons and/or support cells, and much weaker expression in seam cells. (F) Head region of an L1-stage larva showing long processes in GFP-expressing cells. These are likely to be neurons and/or support cells. GFP is also present in seam cells at this stage, but they are not visible in this focal plane. (G,H) Embryos stained with anti-ELT-6 (red) and MH27 (green) at the 1.5-fold (G) and 2.5-fold (H) stages. The staining pattern is similar to the expression pattern of the reporter construct shown in E. The arrowheads point to a seam cell (V2) in B-E,G,H, and the arrows point to cells in the nervous system. | |
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| Crop | Crop_picture | WBPicture0000007446 | |
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| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004833 |
