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WBPicture0000007349 | Description | Figure S3. GFP-Fused HCF-1 Is Expressed in the Nucleus of Somatic and Germline Cells (A) The images show the GFP expression of live transgenic worms carrying low-copy number of the hcf-1::gfp transgene (rwIs3[Phcf-1::hcf-1::gfp, unc-119]) at different developmental stages under normal culture condition. HCF-1::GFP is expressed in the nucleus of somatic and germline cells. Arrowhead indicates the high levels of autofluorescence observed in the intestine of the adult worm (bottom left panel). (B) hcf-1::gfp worms were fixed and immunostained using anti-GFP. HCF-1::GFP is expressed in the nucleus of somatic (upper panel) and germline cells (bottom panel). DAPI staining was used to indicate the nucleus. Photos were taken at 400x magnification. Arrowhead indicates the autofluorescence observed in the intestine. | |||
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Name | journal.pbio.0060233.sg003.jpg | ||||
Depict | Expr_pattern | Expr8379 | |||
Expr8380 | |||||
Anatomy | WBbt:0004017 | ||||
Cellular_component | GO:0005634 | ||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
Publication_year | 2008 | ||||
Article_URL | DOI | id | 10.1371/journal.pbio.0060233 | ||
Journal_URL | PLoSBiology | ||||
Publisher_URL | PLoS | ||||
Reference | WBPaper00032232 |