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| WBPicture0000007319 | Description | Figure 4. NCA-1, UNC-80, and UNC-79 Are Expressed in the Nervous System, Enriched at Nonsynaptic Regions Along Axons (A) A transcriptional GFP reporter driven by the nca-1 promoter (left panels) or unc-80 promoter (right panels) is active in neurons in the nerve ring (NR), and ventral nerve cord (VNC) motoneurons. Activity of the nca-1 promoter is also seen in the HSN neuron whereas the unc-80 promoter has activity in the vulval muscles (VM). (B) An antibody against NCA-1 showed specific staining (arrowheads) in the nerve ring (NR) and along nerve cords in wild-type (top left panel) animals that was absent in nca-1(tm1851) deletion mutants (bottom left panel). Similarly, anti-RFP antibody showed specific immunoreactivity in the nerve ring (NR) of UNC-80::RFP (hpIs98) expressing animal (top middle panel) but not in wild-type animals not carrying the transgene (bottom middle panel). * ns: nonspecific staining persisted in negative controls where animals do not express NCA-1 or UNC-80::RFP protein (out of the focal plane for the nca-1 panel). An antibody against UNC-79 showed specific and similar staining at the nerve ring in wild-type animals (right top panel) that disappeared in unc-79 mutants (right bottom panel). (C) Wild-type animals were co-stained with anti-NCA-1 antibody (red) and anti-UNC-10 (green). (D) unc-80;hpIs98 animals co-stained with anti-RFP antibody (red) and anti-UNC-10 (green) showed poor colocalization. Scale bar: 5um. | |||
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| Name | journal.pbio.0060055.g004.jpg | ||||
| Crop | Crop_picture | WBPicture0000007320 | |||
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| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2008 | ||||
| Article_URL | DOI | id | 10.1371/journal.pbio.0060055 | ||
| Journal_URL | PLoSBiology | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00031592 |
