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WBPicture0000007236DescriptionFigure 3. Expression Pattern of rom-1::nls::gfp Expression pattern of the zhIs5[rom-1::nls::gfprom-1::] transcriptional reporter during vulval development. Images on the left (A, C, E, G, and I) show the corresponding Nomarski pictures with the arrows pointing at the Pn.p cell nuclei and the arrowhead indicating the position of the AC nucleus. (B) A mid L2 larva before vulval induction with uniform rom-1::nls::gfp expression in all the Pn.p cells. (D) An early L3 larva in which rom-1::nls::gfp expression was decreased in all VPCs except P6.p (see text for a quantification of the expression pattern). Note that the nuclei of hyp7 and the Pn.p cells that had fused to hyp7 displayed strong rom-1::nls::gfp expression (P1.p, P2.p, P3.p and P9.p in the example shown). (F) A mid to late L3 larva in which P6.p had generated four descendants. Expression of rom-1::nls::gfp occurred only in the 3rd descendants of P.4.p and P8.p after they fused to hyp7. (H) An L4 larva during vulval invagination. No rom-1::nls::gfp was detectable in the 1st and 2nd descendants of P5.p, P6.p, and P7.p, but the AC and the surrounding uterine cells displayed strong rom-1::nls::gfp expression. (K) A late L2 to early L3 larva following the ablation of the precursors of the somatic gonad. No up-regulation of rom-1::nls::gfp in P5.p, P6.p, or P7.p was observed. The scale bar in (K) is 10 um.
Namejournal.pbio.0020334.g003.jpg
DepictExpr_patternExpr3457
AnatomyWBbt:0006889
WBbt:0006890
WBbt:0006897
WBbt:0008112
WBbt:0008117
WBbt:0008121
WBbt:0008125
WBbt:0008129
WBbt:0008133
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2004
Article_URLDOIid10.1371/journal.pbio.0020334
Journal_URLPLoSBiology
Publisher_URLPLoS
ReferenceWBPaper00024876