WormBase Tree Display for Expression_cluster: WBPaper00041002:HF_3d_2.0mM_Down
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| WBPaper00041002:HF_3d_2.0mM_Down | Description | Gene significantly down-regulated by treatment with 2.0mM of HuminFeed until young adult stage (3 days), with a minimum fold change in gene expression of 0.8. | |
|---|---|---|---|
| Species | Caenorhabditis elegans | ||
| Algorithm | For selection of DEGs, an unpaired t -test was performed followed by a significance analysis of microarray (SAM) test including a calculation that estimates the false discovery rate (FDR). FDR, reducing on the one hand type I errors for null associations, was set to a non-stringent level of <12.5%, mainly to guard from an increase of type II error and also based on findings by Levine et al. (2011), which described 12.5% as most acceptable optimum level of FDR, representing the 90th percentile of the normal distribution curve. DEGs exceeding a fold change of 1.25 were further analyzed with respect to their functional clustering. This fold-cut-off was chosen to allow an interpretation that is biologically meaningful, akin to the notion that data of sound technical and experimental quality which returns strong, statistically significant, absolute signal intensities is sufficiently robust to justify a fold-cut-off of >1.2. This analysis was conducted using the functional annotation clustering tool of the Database for Annotation, Visualization, and Integrated Discovery (DAVID; Huang et al., 2007). | ||
| Reference | WBPaper00041002 | ||
| Microarray_results (2214) | |||
| Gene (1882) | |||
| Attribute_of | Microarray_experiment (27) | ||
| Regulation | Regulated_by_treatment | Treatment with 2.0mM of HuminFeed until young adult stage (3 days). | |
| Regulated_by_molecule | WBMol:00002927 | ||
| Associated_with | Life_stage | WBls:0000057 |
