WormBase Tree Display for Expression_cluster: WBPaper00040210:Chlorpyrifos_Diazinon_24C_regulated

WBPaper00040210:Chlorpyrifos_Diazinon_24C_regulated Description: Genes with differential expression under 0.5mg/l Chlorpyrifos (CPF) and 0.5mg/l Diazinon (DZN) treatment at 24 centigrade.
  Species: Caenorhabditis elegans
  Algorithm: To identify the differentially expressed genes in each treatment authors used linear models per toxicant and temperature (gene expression = Toxicant (effect) + error). The lm function in R stats package was used to implement the linear models analysis with recommended default options. For threshold determination authors used a permutation approach. For each of the 23,232 permutations used authors randomly picked a transcript (array spot), which could only be picked once. Authors combined all the expression values of this transcript and randomly distributed them over the replicates and used them in the linear model. In this way authors obtained a threshold for each of the toxicants. Authors used a -log10 p-value 2 as common threshold for the analysis, which resembles to the following FDR per toxicant: 0.0155 for CPF at 24 centigrade, 0.0148 for DZN at 24 centigrade, 0.0168 for CPF+DZN at 24 centigrade, 0.0142 for CPF at 16 centigrade, 0.0151 for DZN at 16 centigrade, and 0.0148 for CPF+DZN, at 16 centigrade.
  Reference: WBPaper00040210
  Microarray_results (1832)
  Gene (1689)
  Attribute_of: Microarray_experiment: WBPaper00040210:Control_vs_CPF-(0.5)_DZN-(1)_Rep1
      WBPaper00040210:Control_vs_CPF-(0.5)_DZN-(1)_Rep2
      WBPaper00040210:Control_vs_CPF-(0.5)_DZN-(1)_Rep3
      WBPaper00040210:CPF-(0.5)_DZN-(1)_vs_Control_Rep1
      WBPaper00040210:CPF-(0.5)_DZN-(1)_vs_Control_Rep2
      WBPaper00040210:CPF-(0.5)_DZN-(1)_vs_Control_Rep3
  Regulation: Regulated_by_molecule: WBMol:00003591
      WBMol:00004357
  Associated_with: Life_stage: WBls:0000038