WormBase Tree Display for Interaction: WBInteraction000538684

WBInteraction000538684 Interaction_type: Regulatory: Change_of_expression_level
  Interactor: Interactor_overlapping_gene: WBGene00001948 Interactor_type: Trans_regulator
      WBGene00003515 Interactor_type: Trans_regulated
      WBGene00006820 Interactor_type: Trans_regulated
      WBGene00006827 Interactor_type: Trans_regulated
      WBGene00003930 Interactor_type: Trans_regulated
  Interaction_summary: "In almost all treatment paradigms, over-expression of hlh-1 resulted in widespread myogenesis; most cells had adopted a muscle-like fate. The muscle marker routinely used to assay myogenesis was myosin heavy chain A [MHC A (Miller et al., 1986) Fig. 1]. Although high levels of MHC A were detected, filaments were disorganized and these muscle-like cells fail to contract. To determine the extent to which these cells adopted a true muscle-like fate, we tested several additional muscle markers, including several structural proteins. The muscle-like cells resulting from heat shock-induced HLH-1 activated a myo-3::gfp reporter gene (Fire and Waterston, 1989) and were positive for filamentous actin, UNC-89 (Benian et al., 1996), UNC-98 (Mercer et al., 2003) and PAT-3 (Gettner et al., 1995; Francis and Waterston, 1985) (data not shown)."
  Detection_method: Reporter_gene: myo-3::GFP
  Regulation_level: Transcriptional
  Regulation_result: Positive_regulate: Life_stage: WBls:0000003
      Anatomy_term: WBbt:0006804
  Paper: WBPaper00025033