WormBase Tree Display for Interaction: WBInteraction000524886
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WBInteraction000524886 | Interaction_type | Physical | ProteinDNA | ||
---|---|---|---|---|---|
Interactor | Interactor_overlapping_gene | WBGene00003100 | Interactor_type | Target | |
Feature_interactor | WBsf977964 | Interactor_type | Bait | ||
Interaction_summary | MAB-3 binds the minimal 247 bp vit- 2 promoter in vitro in a gel-mobility shift assay (Fig. 5, lanes 1 and 2). To test whether MAB-3 binds the site identified by sequence analysis, we made two mutant promoters, vit-2 M1 and vit-2 M2, which have two and five nucleotide changes in the potential MAB-3 binding site, respectively. MAB-3 does not bind to either of the mutant promoters (Fig. 5, lanes 3 and 4), demonstrating that the site is required for MAB-3 binding in vitro. | ||||
Detection_method | Electrophoretic_mobility_shift_assay | ||||
Throughput | Low_throughput | ||||
Paper | WBPaper00003452 |