WormBase Tree Display for Interaction: WBInteraction000521377
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| WBInteraction000521377 | Interaction_type | Physical | ProteinProtein | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00015007 | Interactor_type | Bait | |
| WBGene00000106 | Interactor_type | Target | |||
| Interaction_summary | Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs. | ||||
| Detection_method | Affinity_capture_MS | ||||
| Throughput | Low_throughput | ||||
| WBProcess | WBbiopr:00000007 | ||||
| Paper | WBPaper00031252 | ||||
| Antibody_remark | anti-GFP antibody |
