WormBase Tree Display for Interaction: WBInteraction000520185
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| WBInteraction000520185 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00003514 | Interactor_type | Trans_regulated | |
| Expr_pattern | Expr1537 | ||||
| Transgene | WBTransgene00000380 | ||||
| WBTransgene00000381 | |||||
| Construct | WBCnstr00000380 | ||||
| WBGene00000899 | Interactor_type | Trans_regulator | |||
| Variation_interactor | WBVar00088906 | Interactor_type | Trans_regulator | ||
| Interaction_summary | C183::gfp (myo-2 enhancer) reporter exhibited strong GFP expression in the pharynx from mid- embryogenesis through adulthood. However, the level, pattern and timing of GFP expression in cuIs2; daf-3(mg90) was indistinguishable from that in +/+; cuIs2 throughout development. The strong reduction in GFP expression observed in daf-4(m72) single mutants was largely suppressed in the daf-4(m72); daf-3(mg90) double mutant. As adults, 84% of daf-4(m72); daf- 3(mg90) animals bearing cuIs2 exhibited much stronger GFP expression than daf-4 single mutants. Thus the reduction of C183 enhancer activity observed in daf-4(m72) requires wild-type daf-3. Taken together the results indicate that daf-3 is a repressor of C183 enhancer activity and, as with its dauer-promoting activity, daf-3's transcriptional repressor activity is negatively regulated by daf-4. | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [myo-2 C subelement C183::gfp] | ||||
| Transgene | |||||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Negative_regulate | Anatomy_term | WBbt:0005451 | ||
| Unaffiliated_construct | WBCnstr00000381 | ||||
| Paper | WBPaper00003384 | ||||
| Remark | this object was originally curated by textpresso sentences. xiaodong re-curated manually later. |
