WormBase Tree Display for Interaction: WBInteraction000520124
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| WBInteraction000520124 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00003681 | Interactor_type | Trans_regulated | |
| Expr_pattern | Expr2868 | ||||
| Transgene | WBTransgene00018206 | ||||
| Construct | WBCnstr00017594 | ||||
| WBGene00003328 | Interactor_type | Trans_regulator | |||
| Variation_interactor | WBVar00090850 | Interactor_type | Trans_regulator | ||
| Interaction_summary | The level of nhr-91 transcripts was increased in starved mir-235(n4504) L1 larvae, compared with wild type. In fed wild-type animals, significant upregulation of nhr-91 transcripts temporally coincides with downregulation of mature miR-235 after 8 and 10 h of feeding. Similarly, a reporter gene, in which the gfp-pest complimentary DNA was inserted between the promoter and the 3'UTR of nhr-91 (Pnhr-91::gfp- pest::nhr-91 3' UTR), also showed a burst of expression in the hypodermis, where Pmir-235::gfp was also expressed, after 8 to 10h of feeding. The level of nhr-91 transcripts was further increased in mir-235 mutants compared with wild-type animals after 8 h of feeding, suggesting that mir-235 functions not only to suppress expression of nhr-91 during L1 diapause, but also to limit the kinetics of its upregulation when feeding. | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [nhr-91::gfp] | ||||
| Transgene | |||||
| RT_PCR | |||||
| Regulation_level | Post_transcriptional | ||||
| Paper | WBPaper00042363 |
