WormBase Tree Display for Construct: WBCnstr00042808
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| WBCnstr00042808 | Summary | GFP::tln-1(W387A) | |
|---|---|---|---|
| Driven_by_gene | WBGene00006771 | ||
| Fusion_reporter | GFP | ||
| Other_reporter | tln-1(W387A) substitution | ||
| Construction_summary | The mutation was induced in N2 wild type worms using the CRISPR/Cas9 system for DNA sequence editing. Briefly, the mixture sgRNA (TLN1W387), co-sgRNA (ZQDP10A), HDR ssDNA template (TLN1W387A), tracrRNA (#1072532), and Cas9 riboprotein (cat. #1081058) were microinjected into the distal arm of the hermaphrodite gonad. F1 animals with Dpy phenotypes were isolated and genotyped for the presence of desired W387A mutations by using the single-worm PCR method. The F2 progeny from the F1 mutants were further screened to isolate homozygous animals. Homozygous animals were outcrossed to N2 males multiple times to remove non-specific targeting. To visualize the TLN-1 distribution, AH3437 GFP::tln-1 was also microinjected with the injection mentioned above with a mixture containing sgRNA (TLN1W387). | ||
| Used_for | Transgene_construct | WBTransgene00033495 | |
| Reference | WBPaper00064778 |
