WormBase Tree Display for Construct: WBCnstr00042487
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| WBCnstr00042487 | Summary | [daf-2a/c::tdTomato] | |
|---|---|---|---|
| Driven_by_gene | WBGene00000898 | ||
| Fusion_reporter | tdTomato | ||
| Construction_summary | [daf-2a/c::tdTomato] splicing reporter. 3 kb of the daf-2 promoter, amplified using primers that introduced a 5' HindIII site and a 3' AgeI (primers 1 and 2), was cloned into pBGY487. A daf-2 genomic fragment corresponding to exon 11 and exon 12, including the intervening intronic sequence with AgeI/KpnI overhangs and an ATG translation site was generated by PCR (primers 3 and 4). This fragment plus a KpnI-TdTomato::unc- 54 3'UTR-SpeI fragment (primers 5 and 6) were cloned into pBGY487 daf-2p cut with AgeI/SpeI in a 3-piece ligation to generate pMGL83 daf-2p::daf-2a/c::tdTomato minigene. | ||
| Used_for | Transgene_construct | WBTransgene00032636 | |
| Reference | WBPaper00059335 |
