WormBase Tree Display for Construct: WBCnstr00040912
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| WBCnstr00040912 | Summary | [unc-86::enu-3.1::GFP] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006818 | ||
| Gene | WBGene00010362 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-86::enu-3.1::GFP] translational fusion. A first PCR product containing 3 kb of the enu-3.1 promoter and the entire gene was made from genomic DNA using elongase polymerase (Thermo Fisher Scientific). A second PCR product containing the gfp gene and the unc-54 3'UTR was made from pPD95.75. A third PCR product was made containing a modified unc-86 promoter containing two copies of the core promoter sequence (a gift from Richard Ikegami and Kazuko Fujisawa). A fusion product of ENU- 3.1::GFP was made by PCR using the first and second reaction products (Hobart 2002). ENU-3.1::GFP driven by the unc-86 promoter was then made by PCR from the first fusion gene product and the third PCR product. | ||
| Used_for | Transgene_construct | WBTransgene00032295 | |
| Reference | WBPaper00051476 |
