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WormBase Tree Display for Construct: WBCnstr00022784

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Name Class

WBCnstr00022784SummaryPhsp-16.2::SKN-1::GFP
Driven_by_geneWBGene00002016
GeneWBGene00004804
Fusion_reporterGFP(S65C)
Type_of_constructCterminal_translational_fusion
Construction_summaryCoding sequence of skn-1 (T19E7.2c.1) gene was amplified from a previously constructed SKN-1 overexpression strain (Maduro et al., 2001) and cloned into pPD118.26 expression vector. SKN-1 ORF is under the control of a heat shock promoter and fused with GFP to visualize SKN-1 expression. Transgenic worms were produced by standard microinjection procedure using myo-3::mCherry as a transgene reporter. To induce SKN-1::GFP over expression, 2-4 cell stage embryos were heat shocked at 30C for 30 to 45 min.
DNA_textATGTACACGGACAGCAATAATAGGAACTTTGATGAAGTCAACCATCAGCATCAACAAGAACAAGATTTCAATGGCCAATCCAAATATGATTATCCACAATTCAACCGTCCAATGGGTCTCCGTTGGCGTGATGATCAACGGATGATGGAGTATTTCATGTCGAATGGTCCAGTAGAAACTGTTCCAGTTATGCCAATACTCACCGAGCATCCACCAGCATCTCCATTCGGTAGAGGACCATCTACAGAACGTCCAACCACATCATCTCGATACGAGTACAGTTCGCCTTCTCTCGAGGATATCGACTTGATTGATGTGCTATGGAGAAGTGATATTGCTGGAGAGAAGGGCACACGACAAGTGGCTCCTGCTGATCAGTACGAATGTGATTTGCAGACGTTGACAGAGAAATCGACAGTAGCGCCACTCACTGCCGAAGAGAATGCTCGATATGAAGATCTTTCGAAAGGATTCTATAATGGATTCTTCGAGTCGTTCAATAACAATCAATATCAGCAGAAACATCAGCAACAACAACGAGAACAAATAAAGACACCAACTCTTGAACATCCAACTCAAAAAGCCGAATTGGAAGATGATCTGTTTGATGAAGATCTTGCTCAGCTTTTCGAGGATGTTTCAAGAGAAGAAGGACAATTGAATCAACTTTTTGATAATAAGCAACAACATCCAGTTATCAATAATGTTTCTCTGTCGGAAGGAATTGTTTATAATCAGGCAAATTTGACCGAGATGCAAGAGATGCGTGATTCCTGCAATCAAGTTTCCATTTCAACAATTCCAACAACATCGACTGCTCAACCAGAGACTTTGTTCAATGTAACCGATTCACAGACTGTCGAACAGTGGCTTCCAACAGAAGTTGTACCAAACGATGTGTTCCCAACATCCAACTACGCCTACATTGGAATGCAAAACGACAGTCTTCAAGCAGTTGTATCAAATGGACAGATTGACTATGATCATTCCTATCAATCCACTGGTCAGACTCCACTGTCTCCTCTCATCATTGGATCTTCAGGACGTCAACAGCAGACTCAAACGAGCCCAGGAAGCGTCACAGTGACTGCAACAGCTACTCAATCGTTGTTCGATCCATATCACTCACAGAGACACTCGTTTAGTGATTGCACTACTGATTCGTCATCAACGTGCTCTCGCCTCTCTTCGGAATCTCCACGATACACGTCAGAGAGCTCAACCGGAACTCACGAGTCTCGTTTCTACGGAAAGTTGGCTCCATCCAGTGGATCACGCTACCAACGATCATCGTCTCCACGTTCATCACAATCTTCGATTAAGATCGCGAGAGTTGTTCCACTGGCCAGCGGACAACGGAAGCGTGGACGTCAATCCAAGGATGAGCAGCTCGCCAGTGACAACGAGCTTCCAGTGTCGGCGTTCCAGATTTCGGAGATGTCATTAAGCGAGTTGCAACAAGTGTTGAAGAACGAGAGTCTCAGCGAGTATCAAAGACAGTTGATTCGCAAGATTCGTCGACGCGGAAAGAACAAGGTTGCTGCCCGCACTTGCCGTCAAAGACGCACGGATCGTCACGACAAGATGTCCCATTACATCTGA
ClonepPD118.26
Used_forTransgene_constructWBTransgene00022997
ReferenceWBPaper00044751
LaboratoryBV