WormBase Tree Display for Construct: WBCnstr00022784
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WBCnstr00022784 | Summary | Phsp-16.2::SKN-1::GFP | |
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Driven_by_gene | WBGene00002016 | ||
Gene | WBGene00004804 | ||
Fusion_reporter | GFP(S65C) | ||
Type_of_construct | Cterminal_translational_fusion | ||
Construction_summary | Coding sequence of skn-1 (T19E7.2c.1) gene was amplified from a previously constructed SKN-1 overexpression strain (Maduro et al., 2001) and cloned into pPD118.26 expression vector. SKN-1 ORF is under the control of a heat shock promoter and fused with GFP to visualize SKN-1 expression. Transgenic worms were produced by standard microinjection procedure using myo-3::mCherry as a transgene reporter. To induce SKN-1::GFP over expression, 2-4 cell stage embryos were heat shocked at 30C for 30 to 45 min. | ||
DNA_text | ATGTACACGGACAGCAATAATAGGAACTTTGATGAAGTCAACCATCAGCATCAACAAGAACAAGATTTCAATGGCCAATCCAAATATGATTATCCACAATTCAACCGTCCAATGGGTCTCCGTTGGCGTGATGATCAACGGATGATGGAGTATTTCATGTCGAATGGTCCAGTAGAAACTGTTCCAGTTATGCCAATACTCACCGAGCATCCACCAGCATCTCCATTCGGTAGAGGACCATCTACAGAACGTCCAACCACATCATCTCGATACGAGTACAGTTCGCCTTCTCTCGAGGATATCGACTTGATTGATGTGCTATGGAGAAGTGATATTGCTGGAGAGAAGGGCACACGACAAGTGGCTCCTGCTGATCAGTACGAATGTGATTTGCAGACGTTGACAGAGAAATCGACAGTAGCGCCACTCACTGCCGAAGAGAATGCTCGATATGAAGATCTTTCGAAAGGATTCTATAATGGATTCTTCGAGTCGTTCAATAACAATCAATATCAGCAGAAACATCAGCAACAACAACGAGAACAAATAAAGACACCAACTCTTGAACATCCAACTCAAAAAGCCGAATTGGAAGATGATCTGTTTGATGAAGATCTTGCTCAGCTTTTCGAGGATGTTTCAAGAGAAGAAGGACAATTGAATCAACTTTTTGATAATAAGCAACAACATCCAGTTATCAATAATGTTTCTCTGTCGGAAGGAATTGTTTATAATCAGGCAAATTTGACCGAGATGCAAGAGATGCGTGATTCCTGCAATCAAGTTTCCATTTCAACAATTCCAACAACATCGACTGCTCAACCAGAGACTTTGTTCAATGTAACCGATTCACAGACTGTCGAACAGTGGCTTCCAACAGAAGTTGTACCAAACGATGTGTTCCCAACATCCAACTACGCCTACATTGGAATGCAAAACGACAGTCTTCAAGCAGTTGTATCAAATGGACAGATTGACTATGATCATTCCTATCAATCCACTGGTCAGACTCCACTGTCTCCTCTCATCATTGGATCTTCAGGACGTCAACAGCAGACTCAAACGAGCCCAGGAAGCGTCACAGTGACTGCAACAGCTACTCAATCGTTGTTCGATCCATATCACTCACAGAGACACTCGTTTAGTGATTGCACTACTGATTCGTCATCAACGTGCTCTCGCCTCTCTTCGGAATCTCCACGATACACGTCAGAGAGCTCAACCGGAACTCACGAGTCTCGTTTCTACGGAAAGTTGGCTCCATCCAGTGGATCACGCTACCAACGATCATCGTCTCCACGTTCATCACAATCTTCGATTAAGATCGCGAGAGTTGTTCCACTGGCCAGCGGACAACGGAAGCGTGGACGTCAATCCAAGGATGAGCAGCTCGCCAGTGACAACGAGCTTCCAGTGTCGGCGTTCCAGATTTCGGAGATGTCATTAAGCGAGTTGCAACAAGTGTTGAAGAACGAGAGTCTCAGCGAGTATCAAAGACAGTTGATTCGCAAGATTCGTCGACGCGGAAAGAACAAGGTTGCTGCCCGCACTTGCCGTCAAAGACGCACGGATCGTCACGACAAGATGTCCCATTACATCTGA | ||
Clone | pPD118.26 | ||
Used_for | Transgene_construct | WBTransgene00022997 | |
Reference | WBPaper00044751 | ||
Laboratory | BV |