WormBase Tree Display for Construct: WBCnstr00021194
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WBCnstr00021194 | Summary | [odr-1::GCY-12::GFP] | |
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Driven_by_gene | WBGene00003848 | ||
Gene | WBGene00001538 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [odr-1::GCY-12::GFP] translational fusion. First, the GCY-12::GFP expression vector was made by inserting a gcy-12 cDNA (obtained from cDNA clone, yk316d1, provided by Dr Y. Kohara, National Institute of genetics, Japan. The 5'part was supplemented with a product of 5'RACE) into pPD95.77 in frame so that GFP was fused to the C-terminus of the GCY-12 protein. Then, a 2.4 kb odr-1 promoter region was inserted into the GCY-12::GFP expression vector. | ||
Clone | pPD95.77 | ||
Used_for | Transgene_construct | WBTransgene00031842 | |
Reference | WBPaper00048635 |