WormBase Tree Display for Construct: WBCnstr00020818
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| WBCnstr00020818 | Summary | [unc-115::gfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006839 | ||
| Gene | WBGene00006839 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-115::gfp] translational fusion. The unc-115 cDNA::gfp minigene was constructed by cloning the 1.5-kb neuron-specific unc-115 promoter upstream of the unc-115 cDNA fused in frame to gfp at the 3' end in the pPD95.77 vector. The unc-115 cDNA minigene was generated by removing gfp sequences from the unc-115 cDNA::gfp transgene and replacing them with the natural 3' end of the unc-115 cDNA, including the natural stop codon. The unc-115 minigenes (gfp tagged and natural 3' end) were constructed by replacing an internal BglII fragment of the unc-115 cDNA with the same fragment from unc-115 genomic DNA, which included introns 4 to 10. | ||
| Clone | pPD95.77 | ||
| Used_for | Transgene_construct | WBTransgene00031826 | |
| Reference | WBPaper00025223 |
