WormBase Tree Display for Construct: WBCnstr00020712
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| WBCnstr00020712 | Summary | [cyk-4C-deletion::gfp Cbr-unc-119(+)] | |
|---|---|---|---|
| Gene | WBGene00000875 | ||
| Fusion_reporter | GFP | ||
| Selection_marker | Cbr-unc-119(+) | ||
| Construction_summary | To generate the cyk-4::gfp strains by Mos1-mediated single-copy insertion (MosSCI) a 4.5-kb cyk-4 genomic fragment was amplified with a primer pair, 5'-AATCAGGGCCCTCACAAACACAGCACTCGGTC-3' and 5'-ACTATCCCGGGTACTCACCGGAAACGGAGTC-3' and cloned into the PspOMI/XmaI site of the pBluescript vector. Following insertion of the gfp sequence between the coding region and the stop codon, the BsiWI/XmaI fragment (from 990 bp upstream of the start codon to 1,072 bp downstream of the stop codon) was subcloned into a MosSCI targeting vector, pCFJ178. The deletion was introduced with primer pairs, 5'-TGCAGATGTCCCTAGGAATCGTTGCCAATATAC-3' and 5'-CATCTGCTGGGGTCGATGTTCCACGAT-3'('Tail'). | ||
| Used_for | Transgene_construct | WBTransgene00021113 | |
| WBTransgene00021116 | |||
| Reference | WBPaper00047056 |
