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WormBase Tree Display for Construct: WBCnstr00020472

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Name Class

WBCnstr00020472Summary[ser-2prom3::RSP-6::myr-mCherry]
Driven_by_geneWBGene00004777
GeneWBGene00004703
Fusion_reportermCherry
Type_of_constructTranslational_fusion
Construction_summary[ser-2prom3::RSP-6::myr-mCherry]. The cDNA was PCR-amplified without stop codons and was cloned in pDONR221 using Gateway BP Clonase II (Invitrogen). The ser-2 promoter 3 fragment (Tsalik and Hobert 2003) was PCR-amplified and cloned into pDONR P4-P1r and the GFP coding sequence with the let-858 3' UTR from pPD117.01 was PCRamplified and cloned into pDONR P2r-P3 using Gateway BP Clonase II (Invitrogen). The cDNAs, ser-2 promoter 3, and GFP were all cloned into pDEST R4-R3 using Gateway LR Clonase II Plus (Invitrogen). Primers: attB1 - atggacgccaaggtgtacgtcg attB2 - gtgcggagaagcagaacggctgc.
ClonepDEST R4-R3
Used_forTransgene_constructWBTransgene00031782
ReferenceWBPaper00046432