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WormBase Tree Display for Construct: WBCnstr00020417

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Name Class

WBCnstr00020417Summary[prsp-6::GFP]
Driven_by_geneWBGene00004703
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summary[prsp-6::GFP] transcriptional fusion. Presumptive promoter regions were PCR-amplified and subcloned into the pDONR221 vector using Gateway BP Clonase II (Invitrogen). Promoters were then subcloned into pDJK237, a promoterless plasmid with a Gateway cassette upstream of GFP with a 3' UTR from let-858 derived from pPD117.01, using Gateway LR Clonase II Plus (Invitrogen). attB1 - gtgtaaatgtgatgcattcgag attB2 - actgaaaaatcaaattaatttatg.
Used_forTransgene_constructWBTransgene00031772
ReferenceWBPaper00046432