WormBase Tree Display for Construct: WBCnstr00020314
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WBCnstr00020314 | Summary | [UNC-70(N-TSMod)] | |
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Driven_by_gene | WBGene00006803 | ||
Gene | WBGene00006803 | ||
Type_of_construct | Nterminal_translational_fusion | ||
Construction_summary | An N-terminal fusion of TSMod (tension sensor) to UNC-70 was made as follows: TSMod was amplified by PCR using primers MK42F and MK43R flanked by SpeI and SalI restriction sites and inserted after the first 15 bp of unc-70 cDNA under the control of the endogenous unc-70 promoter. The TSMod and unc-70 sequences were separated by a flexible linker consisting of five glycines. | ||
Used_for | Transgene_construct | WBTransgene00020763 | |
Reference | WBPaper00044891 |