Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Construct: WBCnstr00020314

expand all nodes | collapse all nodes | view schema

Name Class

WBCnstr00020314Summary[UNC-70(N-TSMod)]
Driven_by_geneWBGene00006803
GeneWBGene00006803
Type_of_constructNterminal_translational_fusion
Construction_summaryAn N-terminal fusion of TSMod (tension sensor) to UNC-70 was made as follows: TSMod was amplified by PCR using primers MK42F and MK43R flanked by SpeI and SalI restriction sites and inserted after the first 15 bp of unc-70 cDNA under the control of the endogenous unc-70 promoter. The TSMod and unc-70 sequences were separated by a flexible linker consisting of five glycines.
Used_forTransgene_constructWBTransgene00020763
ReferenceWBPaper00044891