WormBase Tree Display for Construct: WBCnstr00019504
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| WBCnstr00019504 | Summary | [spe-8::GFP] | |
|---|---|---|---|
| Driven_by_gene | WBGene00004962 | ||
| Gene | WBGene00004962 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Cterminal_translational_fusion | ||
| Construction_summary | This construct includes 838 bp of the sequence upstream of the start codon and 1039 bp downstream of the stop codon. The GFP coding sequence from plasmid pPD95.75 (Fire Lab C. elegans vector kit obtained from Addgene) was inserted either at the 3' end of the genomic spe-8 sequence. The stop codon was eliminated in spe-8. The various segments of the construct were amplified by PCR using Phusion High Fidelity DNA Polymerase (Thermo Scientific), and they were combined following the PCR fusion technique described by Hobert. | ||
| Used_for | Transgene_construct | WBTransgene00020106 | |
| Reference | WBPaper00045487 |
