WormBase Tree Display for Construct: WBCnstr00019381
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WBCnstr00019381 | Public_name | pTH22.1 | |
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Summary | [Punc-49::unc-49-RFP] | ||
Driven_by_gene | WBGene00006784 | ||
Gene | WBGene00006784 | ||
Fusion_reporter | tagRFP | ||
Construction_summary | A unc-49B-tagRFP minigene composed of 4,070 bp of unc-49 promoter, a genomic DNA fragment containing the first four exons of unc-49, a cDNA fragment corresponding to the last eight exons of unc-49B isoform and the 3' UTR sequence of unc-54. The tagRFP sequence was inserted in the TM3-TM4 intracellular loop of the UNC-49B subunit. The unc-49B-tagRFP minigene fused with the 3' UTR sequence of unc-54 was inserted into pCFJ151 by using SbfI and SpeI sites. | ||
Used_for | Transgene_construct | WBTransgene00020056 | |
Reference | WBPaper00045348 |