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WormBase Tree Display for Construct: WBCnstr00019381

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Name Class

WBCnstr00019381Public_namepTH22.1
Summary[Punc-49::unc-49-RFP]
Driven_by_geneWBGene00006784
GeneWBGene00006784
Fusion_reportertagRFP
Construction_summaryA unc-49B-tagRFP minigene composed of 4,070 bp of unc-49 promoter, a genomic DNA fragment containing the first four exons of unc-49, a cDNA fragment corresponding to the last eight exons of unc-49B isoform and the 3' UTR sequence of unc-54. The tagRFP sequence was inserted in the TM3-TM4 intracellular loop of the UNC-49B subunit. The unc-49B-tagRFP minigene fused with the 3' UTR sequence of unc-54 was inserted into pCFJ151 by using SbfI and SpeI sites.
Used_forTransgene_constructWBTransgene00020056
ReferenceWBPaper00045348