WormBase Tree Display for Construct: WBCnstr00019018
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WBCnstr00019018 | Summary | [punc-86::zfp-1(long isoform)::gfp; myo-2::gfp] | |
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Driven_by_gene | WBGene00006818 | ||
Gene | WBGene00006975 | ||
Fusion_reporter | GFP | ||
Construction_summary | A zfp-1(long isoform) complementary DNA (cDNA) was obtained by PCR with N2 cDNA as a template with the forward primer containing the BalI site 5'- ATGAAGAAGTGGCCAATGAAGGAGATGGTAGGTGGATGC-3 and the reverse primer containing the AgeI site 5'-CAAGTTATTGGTTACCGGTCCTTTTCCATTCGGAGTTGCAGATG-3'. A 5.1-kb fragment upstream of the unc-86 transcriptional start site was isolated by PCR from genomic DNA with the forward primer containing the SphI site 5'-CGTGACACTGCATGCTTCAAAAACTGTCAACTAACAAGAT-3' and the reverse primer containing the SalI site 5'-CGGATGCGGTTGTCGACTCATTCAATTTCACTTTTTCATTCG-3'. The zfp-1 cDNA and the unc-86 promoter were subcloned into the Fire Kit GFP vector pPD95.75. The resulting transgene was injected at 0.5 ng/ul with the co-injection marker pPD118.33 (myo-2::gfp) at 4 ng/ul. | ||
Used_for | Transgene_construct | WBTransgene00019687 | |
Reference | WBPaper00044844 |