Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Construct: WBCnstr00019018

expand all nodes | collapse all nodes | view schema

Name Class

WBCnstr00019018Summary[punc-86::zfp-1(long isoform)::gfp; myo-2::gfp]
Driven_by_geneWBGene00006818
GeneWBGene00006975
Fusion_reporterGFP
Construction_summaryA zfp-1(long isoform) complementary DNA (cDNA) was obtained by PCR with N2 cDNA as a template with the forward primer containing the BalI site 5'- ATGAAGAAGTGGCCAATGAAGGAGATGGTAGGTGGATGC-3 and the reverse primer containing the AgeI site 5'-CAAGTTATTGGTTACCGGTCCTTTTCCATTCGGAGTTGCAGATG-3'. A 5.1-kb fragment upstream of the unc-86 transcriptional start site was isolated by PCR from genomic DNA with the forward primer containing the SphI site 5'-CGTGACACTGCATGCTTCAAAAACTGTCAACTAACAAGAT-3' and the reverse primer containing the SalI site 5'-CGGATGCGGTTGTCGACTCATTCAATTTCACTTTTTCATTCG-3'. The zfp-1 cDNA and the unc-86 promoter were subcloned into the Fire Kit GFP vector pPD95.75. The resulting transgene was injected at 0.5 ng/ul with the co-injection marker pPD118.33 (myo-2::gfp) at 4 ng/ul.
Used_forTransgene_constructWBTransgene00019687
ReferenceWBPaper00044844