WormBase Tree Display for Construct: WBCnstr00018210
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| WBCnstr00018210 | Other_name | Expr11193_Ex | |
|---|---|---|---|
| Summary | [Punc-86::GFP::RIN-1] | ||
| Driven_by_gene | WBGene00006818 | ||
| Gene | WBGene00008183 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | Full-length rin-1 cDNA was generated by ligating several partial cDNA fragments derived from yk clones (gifts from Y. Kohara, NIG, Japan); missing sequences in uncovered regions were obtained using RT-PCR. The resulting full-length cDNA was fully sequenced, and its exon and intron organization checked. For the rescue experiments, the full-length cDNA fragment was subcloned between the SmaI and SpeI sites of pPD95.77 and a GFP fragment was inserted into the SmaI site, generating an N-terminal fusion GFP::RIN-1. unc-86 HSN neuron specific promoter was inserted upstream of the GFP sequence. | ||
| Used_for | Transgene_construct | WBTransgene00018849 | |
| Reference | WBPaper00043985 |
