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WormBase Tree Display for Construct: WBCnstr00018174

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Name Class

WBCnstr00018174Other_nameExpr11126_Ex
Summary[R09B5.11::GFP]
Driven_by_geneWBGene00019979
GeneWBGene00019979
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summaryThe R09B5.11 promoter sequence was amplified from the C.elegans genomic DNA with the primer set R09up-5sph (5'CATGCATGCTTCCGGACTAGCTAGCATAC3') and R09up-3 (5'CTATCATTTTTATTTTGACTGGCAAC3'), and the full-length cDNA sequence was amplified from the pCR-R09 plasmid with the primer set R09-5utr (5'CAAAGGCTAGACATTCTATAC3') and R09cdna-3sma (5'GGGTGAACATATCCGTTTTCTCATG3'). These amplicons were used as the templates for overlap extension PCR amplification of the DNA fragment containing both the promoter and cDNA by the primer set R09up-5sph and R09cdna-3sma. This amplicon and the pPD95.79 vector were digested with SphI and SmaI and were then ligated to form the C. elegans expression plasmid of R09B5.11 (pPD-R09g). --precise ends.
Used_forTransgene_constructWBTransgene00018813
ReferenceWBPaper00042558