WormBase Tree Display for Construct: WBCnstr00015012
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| WBCnstr00015012 | Other_name | Expr10031_Ex | |
|---|---|---|---|
| Summary | [pat-9::gfp] | ||
| Driven_by_gene | WBGene00003933 | ||
| Gene | WBGene00003933 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [pat-9::gfp] translational fusion. pat-9::gfp fusion was constructed as follows. First, the 3.3 kb pat-9 promoter was PCR-amplified using primers BW-613 and BW- 630. This fragment was digested with PstI and KpnI, and then replaced the myo-3 promoter of pPD118.20 (Addgene, Fire Lab C. elegans Vector Kit), to make Ppat-9::gfp. The pat-9 genomic fragment including 980 bp downstream of the 6th exon was PCR-amplified using primers BW631 and BW596, digested with KpnI and DraIII, and then replaced the KpnI - DraIII region of Ppat-9::gfp, to make Ppat-9::pat-9. Finally, gfp was amplified and digested with KpnI, and inserted into Ppat-9::pat-9, to make Ppat-9::pat-9::gfp. The pat-9 promoter and genomic coding region were amplified using cosmid T27b1 as a template; gfp was amplified using vector pPD118.20 as a template. | ||
| Clone | pPD118.20 | ||
| T27B1 | |||
| Used_for | Transgene_construct | WBTransgene00031338 | |
| Reference | WBPaper00041122 |
