WormBase Tree Display for Construct: WBCnstr00014829
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| WBCnstr00014829 | Summary | [flag::prg-1, cb-unc-119(+)] | |
|---|---|---|---|
| Gene | WBGene00004178 | ||
| Construction_summary | This construct was made as follows: a 5.0 kb PCR fragment containing the genomic sequence of prg-1 with 1 kb upstream and downstream was cloned into pCR-Blunt-II-TOPO using the Zero-Blunt-TOPO cloning kit (Invitrogen). A unique NotI site was removed from the plasmid and reintroduced immediately after the ATG of prg-1, and the 3xflag sequence was inserted into the NotI site. The fragment was subcloned into the MosSCI vector pCFJ151 (Frokjaer-Jensen et al., 2008) using SpeI and XhoI sites. | ||
| Used_for | Transgene_construct | WBTransgene00015234 | |
| Reference | WBPaper00041240 |
