WormBase Tree Display for Construct: WBCnstr00014804
expand all nodes | collapse all nodes | view schema
WBCnstr00014804 | Summary | [Pdhs-16::dhs-16::gfp; lin-15(+)] | |
---|---|---|---|
Driven_by_gene | WBGene00000979 | ||
Gene | WBGene00000979 | ||
Fusion_reporter | GFP | ||
Construction_summary | For this construct a 3.9 kb fragment containing the dhs-16 coding region and 2.3 kb upstream promoter region was amplified with primers 5'-GCGGCCGCCTTCTCTCTTGCACCCTTGTTTGT-3' (forward) and 5'-GGTACCTCAGAAACTGTAACATTATG-3' (reverse) and cloned into pCRIITOPO vector. KpnI/NotI-digested dhs-16-TOPO was then inserted into the KpnI/NotI-digested gfp vector L3871 (Fire vector kit 1997). The construct was injected with the lin-15[+] marker | ||
Used_for | Transgene_construct | WBTransgene00015208 | |
Interactor | WBInteraction000518464 | ||
Reference | WBPaper00040978 |