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WormBase Tree Display for Construct: WBCnstr00014804

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Name Class

WBCnstr00014804Summary[Pdhs-16::dhs-16::gfp; lin-15(+)]
Driven_by_geneWBGene00000979
GeneWBGene00000979
Fusion_reporterGFP
Construction_summaryFor this construct a 3.9 kb fragment containing the dhs-16 coding region and 2.3 kb upstream promoter region was amplified with primers 5'-GCGGCCGCCTTCTCTCTTGCACCCTTGTTTGT-3' (forward) and 5'-GGTACCTCAGAAACTGTAACATTATG-3' (reverse) and cloned into pCRIITOPO vector. KpnI/NotI-digested dhs-16-TOPO was then inserted into the KpnI/NotI-digested gfp vector L3871 (Fire vector kit 1997). The construct was injected with the lin-15[+] marker
Used_forTransgene_constructWBTransgene00015208
InteractorWBInteraction000518464
ReferenceWBPaper00040978