WormBase Tree Display for Construct: WBCnstr00014357
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WBCnstr00014357 | Public_name | fUL#HC40 | |
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Other_name | Expr9837_Ex | ||
Summary | [T28F12.2::GFP; pRF4] | ||
Driven_by_gene | WBGene00006796 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | Clone = fUL#HC40. The reporter gene fusion assayed was made by recombineering WRM061dC01. Sequence of homology arms on primers used to direct site of reporter insertion during recombineering, sequence for upstream of reporter first: cagtagatgctctttctcaacaatgggttgatctctcagcaccacacgaa, aggagaacctttggcggtatactgttgtaataaatatgttcggaccctta. gfp was inserted immediately upstream of the unc-62 stop codon. This reporter gene fusion was also constructed from WRM069bE03, with less upstream and more downstream genomic DNA (see fUL#HC19). Other strains: UL4081 and UL4080. pRF4 cotransformants: pick rollers to maintain (not integrated). --precise ends. | ||
Used_for | Transgene_construct | WBTransgene00031282 | |
Reference | WBPaper00040230 | ||
Person | WBPerson266 |